Ccsb-broad
WebOct 14, 2024 · Dana-Farber Cancer Institute and The Broad Institute provide the CCSB-Broad Lentiviral Expression Library for over 15,000 human ORFs in an expression-ready lentiviral system with a C-terminal V5 tag. Ebright RY et al infected isolated circulating tumor cells with several constructs from the library [ 60 ]. WebFull list of CCSB-Broad hORFeome V8.1 Entry clone id Expression clone id (LR success) Sequence method: Illumina (I), 454 (454), Sanger (S), Unsequenced (U) Source ORF Genbank accession MGC Gene id Gene symbol Sequences compare to MGC reference Collection NCBI gene id Gene symbol Transcripts Match type * ...
Ccsb-broad
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WebSep 5, 2024 · Overexpression constructs were obtained from the CCSB-Broad Lentiviral expression Library (cat. no. OHS6085, OHS6087, OHS6269, OHS6270 and OHS6271). Plasmid encoding GFP and tdTomato, cloned into ... WebNov 6, 2024 · The CCSB-Broad Lentiviral Expression Library, generated by researchers in Dana-Farber Cancer Institute and The Broad Institute, is a genome-wide expression-ready lentiviral system for over 15,000 human ORFs with a C-terminal V5 tag. Detection.
WebDo the CCSB-Broad Lentiviral Expression clones have a native stop codon? No, all of the clones in the CCSB-Broad Lentiviral Expression Library contain a C-terminal V5 epitope tag fused to the ORF of interest. There is a stop codon located at the 3' end of the V5 epitope tag (Figure 3). How do I package the CCSB-Broad Lentiviral Expression clones? WebCcsb Broad Lentiviral Expression Library Horfeome V8 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO …
WebCitrus County School District. Our District proudly serves approximately 16,000 students in pre-school through grade 12 across 23 campuses. We are where learning is the expectation and caring is the commitment. Our passionate educators empower students to take charge of their academic and personal success. The school district spans the cities ... WebJun 26, 2011 · Here we report the creation and characterization of two publicly available genome-scale human ORFeome collections: the human ORFeome version 8.1 Entry …
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WebCCSB-Broad Lentiviral Expression Clones (OHS6085) were fully sequenced, and match their accessions with no more than a single mutation within the open reading frame … avito villa skikina temaraWebCCSB-Broad lentiviral kinome ORF expression library. The facility has an ORF expression library of 600 kinases, in a lentiviral vector. Each kinase is V5-tagged at its C-terminus … avito television samsungWebAug 12, 2015 · The SEC14L3 expression plasmid was obtained from CCSB-Broad lentiviral expression library (Thermo Scientific). The open reading frames (ORFs) from these plasmids were transferred to pLOC ... avito voiture ait melloulhttp://www.sinh.cas.cn/jgsz/zcpt/fxcsjspt/202411/W020241129579541142540.pdf avito tetouan xiaomiWebThe Brevard Electronic Court Application (BECA) offers online admittance to court records in accordance with Florida Supreme Court Administrative Order 2014-19 and as changed … avito voiture h100WebDownload scientific diagram Performance of the CCSB-Broad lentiviral expression library. (a) Micrographs show A549 lung cancer cell lines stained with an antibody to the V5 epitope after ... avito toyota hilux marrakechWebA SERPINB9 overexpression construct was obtained from the CCSB-Broad Lentiviral Expression Library (Thermo Fisher) and introduced into tumor cells following the same protocol as described for sgRNAs. Selection was performed with 10 µg/ml blasticidin. Single-cell sequencing. avito villa harhoura