WebMar 22, 2024 · EasyFRAP computes the mobile fraction, defined as the fraction of bleached particles that are mobile (free to diffuse or transiently binding) and therefore the respective fluorescence recovers during the time-course of the experiment, and the half maximal recovery time (t-half), defined as the time at which the recovery of the fluorescence was ... WebeasyFRAP Batch analysis Fitting: 1. Select fitting method 2. Press Fit The data are fitted and the results (mobile fraction, t-half, their means and standard deviations) are saved to .xls files in the parent directory named after the experiments (e.g. Fitting results- test1.xls) 2. Press Plot to plot all fitted curves and residuals
Analysis of Protein Kinetics Using Fluorescence Recovery After ...
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Quantitative diffusion measurements using the open-source
WebJul 1, 2024 · The easyFRAP-web graphical user interface. EasyFRAP-web is designed as a single page application consisting of five progressively activated sections to simplify analysis. (A) Data upload panel. Multiple individual FRAP files can be uploaded (left) and subsequently excluded or restored at any time from the file manager (right table). WebAug 5, 2024 · ImageJ and the EasyFRAP-web tool were used for quantitative analysis of the FRAP measurements . Briefly, fluorescence intensity was normalized using the full-scale method, and last curve fitting was performed using the double term exponential equation to determine the mobile fraction, M f , and the half-maximal recovery time, t 1/2 . WebEasyFRAP-web is a web-based application which facilitates qualitative and quantitative analysis of Fluorecence Recovery After Photobleaching (FRAP) data. It is an easy-to-use software tool that facilitates the fast and interactive analysis of FRAP curves. EasyFRAP-web allows large data-sets to be rapidly evaluated and normalized fluorescence recovery … greetingx.com