Gapdh was used as a loading control
WebMar 20, 2024 · A common approach is the use of a loading control, a constitutively expressed protein that does not vary among treatment groups, to normalize the relative expression of the protein of interest. ... Zeng Q et al used the CST GAPDH antibody ( 2118) as a loading control for the western blot analysis of N-methyl-D-aspartate receptors in … WebMay 16, 2024 · GAPDH was used as the loading control. d The densitometry analysis of PCNP was performed, normalized to the corresponding GAPDH level. e DNA replication activities of TPC-1 and ARO cells in each group were examined by EdU assay; original magnification 100 ×. f The proliferation rate of each group was analysed. g The …
Gapdh was used as a loading control
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WebAntibodies that detect GAPDH can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ELISA. ... Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis. Synonyms. 38 kDa BFA-dependent ADP ... WebNov 9, 2011 · Novus offers a complete selection of highly characterized beta-Actin antibodies. GAPDH (36 kDa) is integral for glycolysis and …
WebGAPDH antibody, is usually used as a loading control antibody for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. OriGene offers high quality of Anti-GAPDH TrueRAB™ antibody (Loading control). View all GAPDH loading control antibodies. WebGAPDH單株抗體可用做loading control antibody。 GAPDH (甘油醛 3-磷酸脫氫酶, Glyceraldehyde 3-phosphate dehydrogenase, 縮寫為 GAPDH 或 G3PDH）是一種 37kDa 的酶，可催化糖酵解的第六步，從而分解葡萄糖(glucose)以獲取能量(energy)和碳分 …
WebApr 14, 2024 · Traditionally, studies of GAPDH have mainly focused on its glycolytic function. With the discovery of GAPDH in cytoplasm, mitochondria, nucleus, and other cell compartments, it has gradually been accepted as a multifunctional protein. For example, GAPDH might act as an intracellular sensor for oxidative stress at the early stage of … WebGAPDH was used as a loading control. To assess the integrity of eIF3, the anti-eIF3b co-immunoprecipitation experiments were carried out for each knock-down and the immunoprecipitated eIF3 subunits along with eIF3b …
WebThis GAPDH antibody loading control is available for research use only. Review this product on BioCompare and get a $20 Amazon gift card Powered by Bioz See more details on Bioz. Related Products ‹ GAPDH Antibody [clone RM114] (R20248) ... GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for ...
WebLoading control antibodies are often detecting housekeeping proteins that are abundantly expressed, leading to signal saturation, particularly when a chemiluminescent detection method is used. Oversaturation may render loading control bands useless for reference, may hide sample-to-sample variation in target protein quantity. laws on growing marijuana in missouriWebWhen it comes to mouse tissue samples, not all western blot loading control proteins are expressed equally. For example, there are relatively low amounts of β-actin in mouse heart and relatively low amounts of GAPDH in mouse small intestine. Plus, under certain experimental conditions, the protein levels of some loading control proteins can be ... laws on guardianshipWebMouse anti Rabbit GAPDH (MCA4739) used as a Western blotting loading control on mouse liver lysates. Image caption: Fibropolycystic disease in aging Jnk Δhepa mice is characterized by extracellular matrix deposition and inflammation. Western Blot of 32 week Jnk f/f and Jnk δhepa mouse liver lysates for αSMA with GAPDH used as a loading … lawson hamilton lewisburg wvWebWhy Choose OriGene Loading Controls. a. OriGene offers a wide selection of loading control antibodies for GAPDH , beta-tubulin and beta-actin and HSP90AB for several species. b. Most are monoclonal antibodies provided under rigorous quality control on a panel of cell lines. c. HRP- and biotin-conjugated versions of these antibodies are also ... kartrite waterpark monticello nyWebJan 14, 2024 · The two most commonly used controls are beta-actin and Glyceraldehyde 3-phosphate dehydrogenase (GADPH). Beta-actin is commonly used as a western blot loading control as is expressed within all ... lawson grocery storeWebApr 4, 2024 · (B) Representative image of the western blotting results for MHC; GAPDH was used as a loading control. ( C ) The bar chart shows the quantification of MHC. ( D ) Myotubes were treated with RSV at a series of concentrations (1, 5, 10, 25 or 50 μM) for 24 h, and cell viability was detected by CCK-8 assay. laws on gun controlWebGAPDH Loading Control Antibody (MA5-15738-BTIN) in WB. Western blot analysis of GAPDH was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated GAPDH ... laws on growing weed in illinois