Jc 1 staining principle
WebMay 9, 2024 · Mitochondrial function was evaluated by JC-1 staining. Double immunofluorescence labeling of LC3 and TOMM20 used to assess the degree of … WebI understand JC-1 staining is used by many people to monitor mitochondrial membrane potential in apoptosis induction in a variety of cell systems. I see also different ways of interpreting the ...
Jc 1 staining principle
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WebJul 7, 2024 · JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions … WebFurthermore, another kind of dye, JC-1, yields green or red fluorescence depending on MMP and allows to distinguish cells with high or low mitochondria potential. Both measuring …
WebJan 1, 2024 · JC-1 can be used to assess the ΔΨMboth in intact isolated mitochondria and tissues. In fact, the JC-1 dye is a lipophilic, cationic dye (naturally exhibiting green fluorescence) which is able to enter into the mitochondria where it accumulates and (in a concentration-dependent manner) starts forming reversible complexes called J aggregates. WebJC-1 Dye for Mitochondrial Membrane Potential Thermo Fisher Scientific - KR Popular TaqMan Real-Time PCR Assays Antibodies Oligos, Primers & Probes GeneArt Gene …
WebJC-1 is a fluorescent compound that accumulates in mitochondria according to their transmembrane potentials. But lox potential mitochondria are usually bigger, and higher … WebNov 22, 2012 · The cyanine dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide) facilitates discrimination of energized and deenergized …
WebJC-1 Staining Solution Preparation Thaw an aliquot of the JC-1 Reagent (Item No. 10009908) at room temperature. Prepare a staining solution by diluting the reagent 1:10 …
WebJun 1, 1998 · The staining intensity of JC-1 in HSC reverted to the levels seen in unseparated bone marrow samples when the staining was performed in the presence of the MDR blockers, suggesting preferential efflux of JC-1 by an MDR mechanism in HSC. corpus christi cleveland ohioWebRemove and incubate for 5 min at 20–22°C. The reaction is initiated by the addition of 50 μL of the dye reagent and allowed to proceed for 30 min. The incubation mixture is then filtered on a 0.2 µm pore filter either through a syringe filter unit or an inserted filter for microcentrifuge tube as shown in Figure 7.1. farcry six co opWeb2. JC-1 Staining Solution (2X) Preparation Thaw JC-1 Reagent (Item No. 10009908) at room temperature. Mix well to ensure there are no particles or flakes in the solution. Make a 2X staining solution by diluting the stock vial 1:100 into complete culture medium (containing FBS), and keep in the dark at room temperature until use. corpus christi clifton parkWebPrepare Staining Solution. Mix 25 µl of the 200× JC-1 Stock Solution in 4 ml of ultrapure water in an appropriate tube. Close the tube and mix the solution by inversion or vortex … corpus christi city officesWebDec 26, 2015 · 5. Wash each staining jar with three (3) changes of 1% Calcium Chloride for a total of approximately ten (10) minutes. 6. Add 2% Cobalt Chloride to each jar for ten (10) minutes. 7. Wash with three (3) to five (5) changes of an approximately 1:20 solution of 0.1M Sodium Barbital (~ 5 ml 0. 1 M Sodium Barbital + deionized water ~ 100 ml). far cry songsWebMay 9, 2024 · In the normal state, JC-1 aggregates in the mitochondrial matrix to form polymers, producing red fluorescence signals; when mitochondrial membrane potentials decrease, JC-1 disaggregates to monomers which produce green fluorescence. Assays were conducted as follows. corpus christi clip artWebWhen excited simultaneously by 488 nm argon-ion laser sources, the JC-1 monomer and J-aggregate can be detected separately in the conventional flow cytometer FL1 and FL2 … corpus christi class of 1993